Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells present in urine. This method is advantageous in many circumstances, as the isolation of urinary cells is simple (30 ml of urine are sufficient), cost-effective and universal (can be applied to any age, gender and race). Moreover, the entire procedure is reasonably quick—around 2 weeks for the urinary cell culture and 3–4 weeks for the reprogramming—and the yield of iPSC colonies is generally high—up to 4% using retroviral delivery of exogenous factors. Urinary iPSCs (UiPSCs) also show excellent differentiation potential, and thus represent a good choice for producing pluripotent cells from normal individuals or patients with genetic diseases, including those affecting the kidney.
Scalable amounts of liver and pancreas precursor cells created using new stem cell production method
Scientists in Canada have overcome a key research hurdle to developing regenerative treatments for diabetes and liver disease with a technique to produce medically useful amounts of endoderm cells from human pluripotent stem cells. The research, published in Biotechnology and Bioengineering, can be transferred to other areas of stem cell research helping scientists to navigate the route to clinical use known as the ‘valley of death’.
“One million people suffer from type 1 diabetes in the United States, while liver disease accounts for 45,000 deaths a year,” said Dr Mark Ungrin from the University of Toronto. “This makes stem cells, and the potential for regenerative treatments, hugely interesting to scientists. Laboratory techniques can produce thousands, or even millions, of these cells, but generating them in the numbers and quality needed for medicine has long been a challenge.”
The research focused on the process of using pluripotent stem cells (PSC) to generate endoderm cells, one of the three primary germ layers which form internal organs including the lungs, pancreas, and liver. The ability to differentiate, or transform, PSCs into endoderm cells is a vital step to developing regenerative treatments for these organs.
Research into treatments that fight male-pattern baldness might take a lesson from animals that beef up their fur coats at certain times of the year.
In animals, hair growth is triggered not only by hormones in the layer of skin called the dermis, but also by signals coming from elsewhere in the body, said study author Dr. Cheng-Ming Chuong, a professor at the University of Southern California.
These signals vary with the seasons, the research showed, which is why some animals lose and gain coats of hair at different times of the year.
“The hair-follicle stem cell is not only listening to the voice in the stem cell, but also the voice from outside,” Chuong explained, in an interview prior to his presentation today at the meeting of the American Society for Cell Biology in Denver.
The search for ways to regrow hair has recently turned to stem cells, but the new study indicates that instead, a treatment could aim at altering the environment around hair follicles, rather than implanting stem cells within them.
These outside signals that are present in animals are missing in people.
“This extra follicle-affecting factor has disappeared during human evolution,” so human hair follicles are activated only by signals internal to the hair follicle, Chuong told MyHealthNewsDaily.
So to regrow hair for men who have lost it with age, a treatment could be developed that targets the tissue around the hair follicle to stimulate hair growth.
“To deal with the hair growth, you not only try to help the stem cell, but you can improve the ‘soil,’ like — You put a tulip bulb in a nicer soil, you will grow a nicer hair,” Chuong said.
Last month, a paper in the Journal of Investigative Dermatology from researchers at the University of California - Irvine noted that this line of research contributes to an understanding of how to trigger hair growth once a follicle is in the “resting phase” of hair growth.
ALS Research News: Grafting of human spinal stem cells into ALS rats best with immunosuppressant combination
Via Scoop.it - ALS Lou Gehrig’s Disease
A team of researchers grafting human spinal stem cells into rats modeled with amyotrophic lateral sclerosis (ALS), also known as “Lou Gehrig’s Disease,” a degenerative, lethal, neuromuscular disease, have tested four different immunosuppressive…
Such a tiny thing to instigate so much moral controversy.
Stem cell research on donor eggs often not disclosed
Many U.S. fertility clinics don’t tell egg donors that embryos made from their eggs may end up being used in stem cell research despite widespread opposition to such research, which is considered morally offensive by a third of Americans.
Via Scoop.it - ALS Lou Gehrig’s Disease
China has ordered a halt to all unapproved stem cell treatments and clinical trials, state media reported on Tuesday, as Beijing seeks to rein in the largely untested stem cell therapies now on offer across the country. The Ministry of Health will stop…